Synthetic teaching example of calcium signal in arbitrary units. Six matched donors; each line joins the same donor before and after stimulation. Horizontal offsets separate points while retaining the pairing and measured y values. Two-sided paired t-test, one planned comparison, no multiplicity correction; unadjusted 95% CI of mean within-donor change. Only P < 0.05 is annotated; the full result is saved. Plot area: 50 x 50 mm; the export includes labels at their original font sizes.
