Direct conversion from H5AD format to Seurat object without intermediate h5Seurat. Supports optional BPCells on-disk matrix loading for large datasets that exceed available memory.
Arguments
- file
Path to H5AD file
- assay.name
Name for the primary assay (default: "RNA")
- use.bpcells
If not NULL, a directory path where BPCells will store the expression matrix on disk. Requires the BPCells package. The resulting Seurat object will reference the on-disk matrix instead of loading it into memory, enabling analysis of datasets larger than available RAM.
- verbose
Show progress messages